ASTM E2048-1999(2006) 用聚合酶链式反应方法探测结核分支杆菌复合物和其它病原分支杆菌的核酸的标准指南
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【英文标准名称】:StandardGuideforDetectionofNucleicAcidsoftheMycobacteriumTuberculosisComplexandOtherPathogenicMycobacteriabythePolymeraseChainReactionTechnique
【原文标准名称】:用聚合酶链式反应方法探测结核分支杆菌复合物和其它病原分支杆菌的核酸的标准指南
【标准号】:ASTME2048-1999(2006)
【标准状态】:现行
【国别】:
【发布日期】:1999
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E48.02
【标准类型】:(Guide)
【标准水平】:()
【中文主题词】:DNA胞质基因;DNA研究;M细胞间;MTB;结核分支杆菌复合物;结核分支杆菌;聚合酶链式反应;PCR;结核病;放大
【英文主题词】:amplification;DNAprimer;DNAprobe;M.avium;M.intercellulare;MTB;MTBcomplex;Mycobacteriumtuberculosis;PCR;polymerasechainreaction;TB;tuberculosis
【摘要】:ThisguideisintendedforuseinanylaboratoryutilizingPCRorRT-PCRtoamplifyanddetectnucleicacidsequencesofmycobacteriafromabiologicalpreparationandtoidentifythespeciesoforigin.ThecriteriausedfortheidentificationandevaluationoftheamplificationreactionsshouldbeadministeredbyanindividualtrainedintheuseofmolecularbiologicalandmicrobiologicaltechniquesassociatedwithPCRandMTB.1.1Thisguidecoversbasicconsiderations,criteria,principlesandrecommendationsthatshouldbehelpfulwhendeveloping,utilizing,orassessingPCR-specificprotocolsfortheamplificationanddetectionoridentificationofmycobacterialnucleicacids.Thisguideisnotaspecificprotocolforthedetectionofspecificmycobacteria.Itisintendedtoprovideinformationthatwillassisttheuserinobtaininghighqualityandreliabledata.TheguideiscloselyrelatedtoandshouldbeusedconcurrentlywiththegeneralPCRGuideE1873.1.2Thisguidehasbeendevelopedforuseinanymolecularbiologyorbiotechnologylaboratory.Itmaybeusefulforthedetectionofmycobacteriainclinical,diagnosticlaboratories.1.3ThisguidedoesnotcoverdetailsofthevariousmethodssuchasgelelectrophoresisthatcanbeutilizedtohelpidentifyPCR-amplifiedmycobacterialnucleicacidsequences,anditdoesnotcoverdetailsofinstrumentcalibration.1.4ThisguidedoesnotcoverspecificvariationsofthebasicPCRorRT-PCRtechnology(forexample,quantitativePCR,multiplexPCRandinsituPCR),anditdoesnotcoverdetailsofinstrumentcalibration.1.5Warning-Laboratoryworkinvolvingcertainclinicalspecimensandmicroorganismscanbehazardoustopersonnel.Precaution:BiosafetyLevel2facilitiesarerecommendedforpotentiallyhazardouswork,andBiosafetyLevel3facilitiesarerequiredforpropagatingandmanipulatingMycobacteriatuberculosiscultures().SafetyguidelinesshouldbeadheredtoaccordingtoNCCLSM29-T2,I17-Pandotherrecommendations().
【中国标准分类号】:C05
【国际标准分类号】:07_100_99
【页数】:9P.;A4
【正文语种】:
【原文标准名称】:用聚合酶链式反应方法探测结核分支杆菌复合物和其它病原分支杆菌的核酸的标准指南
【标准号】:ASTME2048-1999(2006)
【标准状态】:现行
【国别】:
【发布日期】:1999
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E48.02
【标准类型】:(Guide)
【标准水平】:()
【中文主题词】:DNA胞质基因;DNA研究;M细胞间;MTB;结核分支杆菌复合物;结核分支杆菌;聚合酶链式反应;PCR;结核病;放大
【英文主题词】:amplification;DNAprimer;DNAprobe;M.avium;M.intercellulare;MTB;MTBcomplex;Mycobacteriumtuberculosis;PCR;polymerasechainreaction;TB;tuberculosis
【摘要】:ThisguideisintendedforuseinanylaboratoryutilizingPCRorRT-PCRtoamplifyanddetectnucleicacidsequencesofmycobacteriafromabiologicalpreparationandtoidentifythespeciesoforigin.ThecriteriausedfortheidentificationandevaluationoftheamplificationreactionsshouldbeadministeredbyanindividualtrainedintheuseofmolecularbiologicalandmicrobiologicaltechniquesassociatedwithPCRandMTB.1.1Thisguidecoversbasicconsiderations,criteria,principlesandrecommendationsthatshouldbehelpfulwhendeveloping,utilizing,orassessingPCR-specificprotocolsfortheamplificationanddetectionoridentificationofmycobacterialnucleicacids.Thisguideisnotaspecificprotocolforthedetectionofspecificmycobacteria.Itisintendedtoprovideinformationthatwillassisttheuserinobtaininghighqualityandreliabledata.TheguideiscloselyrelatedtoandshouldbeusedconcurrentlywiththegeneralPCRGuideE1873.1.2Thisguidehasbeendevelopedforuseinanymolecularbiologyorbiotechnologylaboratory.Itmaybeusefulforthedetectionofmycobacteriainclinical,diagnosticlaboratories.1.3ThisguidedoesnotcoverdetailsofthevariousmethodssuchasgelelectrophoresisthatcanbeutilizedtohelpidentifyPCR-amplifiedmycobacterialnucleicacidsequences,anditdoesnotcoverdetailsofinstrumentcalibration.1.4ThisguidedoesnotcoverspecificvariationsofthebasicPCRorRT-PCRtechnology(forexample,quantitativePCR,multiplexPCRandinsituPCR),anditdoesnotcoverdetailsofinstrumentcalibration.1.5Warning-Laboratoryworkinvolvingcertainclinicalspecimensandmicroorganismscanbehazardoustopersonnel.Precaution:BiosafetyLevel2facilitiesarerecommendedforpotentiallyhazardouswork,andBiosafetyLevel3facilitiesarerequiredforpropagatingandmanipulatingMycobacteriatuberculosiscultures().SafetyguidelinesshouldbeadheredtoaccordingtoNCCLSM29-T2,I17-Pandotherrecommendations().
【中国标准分类号】:C05
【国际标准分类号】:07_100_99
【页数】:9P.;A4
【正文语种】:
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